Acquisition of cell-cell fusion activity by amino acid substitutions in spike protein determines the infectivity of a coronavirus in cultured cells.
Identifieur interne : 002B91 ( Main/Exploration ); précédent : 002B90; suivant : 002B92Acquisition of cell-cell fusion activity by amino acid substitutions in spike protein determines the infectivity of a coronavirus in cultured cells.
Auteurs : Yoshiyuki Yamada [Singapour] ; Xiao Bo Liu ; Shou Guo Fang ; Felicia P L. Tay ; Ding Xiang LiuSource :
- PloS one [ 1932-6203 ] ; 2009.
Descripteurs français
- KwdFr :
- Animaux, Cellules cultivées, Coronavirus (pathogénicité), Cytométrie en flux, Données de séquences moléculaires, Fusion cellulaire, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (physiologie), Mutagenèse dirigée, Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (physiologie), RT-PCR, Similitude de séquences d'acides aminés, Substitution d'acide aminé, Séquence d'acides aminés, Technique d'immunofluorescence, Technique de Western, Électroporation.
- MESH :
- génétique : Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- pathogénicité : Coronavirus.
- physiologie : Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- Animaux, Cellules cultivées, Cytométrie en flux, Données de séquences moléculaires, Fusion cellulaire, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Mutagenèse dirigée, Protéines de l'enveloppe virale, RT-PCR, Similitude de séquences d'acides aminés, Substitution d'acide aminé, Séquence d'acides aminés, Technique d'immunofluorescence, Technique de Western, Électroporation.
English descriptors
- KwdEn :
- Amino Acid Sequence, Amino Acid Substitution, Animals, Blotting, Western, Cell Fusion, Cells, Cultured, Coronavirus (pathogenicity), Electroporation, Flow Cytometry, Fluorescent Antibody Technique, Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Membrane Glycoproteins (physiology), Molecular Sequence Data, Mutagenesis, Site-Directed, Reverse Transcriptase Polymerase Chain Reaction, Sequence Homology, Amino Acid, Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Envelope Proteins (physiology).
- MESH :
- chemical , chemistry : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- pathogenicity : Coronavirus.
- chemical , physiology : Membrane Glycoproteins, Viral Envelope Proteins.
- Amino Acid Sequence, Amino Acid Substitution, Animals, Blotting, Western, Cell Fusion, Cells, Cultured, Electroporation, Flow Cytometry, Fluorescent Antibody Technique, Molecular Sequence Data, Mutagenesis, Site-Directed, Reverse Transcriptase Polymerase Chain Reaction, Sequence Homology, Amino Acid, Spike Glycoprotein, Coronavirus.
Abstract
Coronavirus host and cell specificities are determined by specific interactions between the viral spike (S) protein and host cell receptor(s). Avian coronavirus infectious bronchitis (IBV) has been adapted to embryonated chicken eggs, primary chicken kidney (CK) cells, monkey kidney cell line Vero, and other human and animal cells. Here we report that acquisition of the cell-cell fusion activity by amino acid mutations in the S protein determines the infectivity of IBV in cultured cells. Expression of S protein derived from Vero- and CK-adapted strains showed efficient induction of membrane fusion. However, expression of S protein cloned from the third passage of IBV in chicken embryo (EP3) did not show apparent syncytia formation. By construction of chimeric S constructs and site-directed mutagenesis, a point mutation (L857-F) at amino acid position 857 in the heptad repeat 1 region of S protein was shown to be responsible for its acquisition of the cell-cell fusion activity. Furthermore, a G405-D point mutation in the S1 domain, which was acquired during further propagation of Vero-adapted IBV in Vero cells, could enhance the cell-cell fusion activity of the protein. Re-introduction of L857 back to the S gene of Vero-adapted IBV allowed recovery of variants that contain the introduced L857. However, compensatory mutations in S1 and some distant regions of S2 were required for restoration of the cell-cell fusion activity of S protein carrying L857 and for the infectivity of the recovered variants in cultured cells. This study demonstrates that acquisition of the cell-cell fusion activity in S protein determines the selection and/or adaptation of a coronavirus from chicken embryo to cultured cells of human and animal origins.
DOI: 10.1371/journal.pone.0006130
PubMed: 19572016
Affiliations:
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Le document en format XML
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Tay</name></author><author><name sortKey="Liu, Ding Xiang" sort="Liu, Ding Xiang" uniqKey="Liu D" first="Ding Xiang" last="Liu">Ding Xiang Liu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19572016</idno><idno type="pmid">19572016</idno><idno type="doi">10.1371/journal.pone.0006130</idno><idno type="wicri:Area/PubMed/Corpus">001868</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001868</idno><idno type="wicri:Area/PubMed/Curation">001868</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001868</idno><idno type="wicri:Area/PubMed/Checkpoint">001951</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001951</idno><idno type="wicri:Area/Ncbi/Merge">001F60</idno><idno type="wicri:Area/Ncbi/Curation">001F60</idno><idno type="wicri:Area/Ncbi/Checkpoint">001F60</idno><idno type="wicri:Area/Main/Merge">002C45</idno><idno type="wicri:Area/Main/Curation">002B91</idno><idno type="wicri:Area/Main/Exploration">002B91</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Acquisition of cell-cell fusion activity by amino acid substitutions in spike protein determines the infectivity of a coronavirus in cultured cells.</title><author><name sortKey="Yamada, Yoshiyuki" sort="Yamada, Yoshiyuki" uniqKey="Yamada Y" first="Yoshiyuki" last="Yamada">Yoshiyuki Yamada</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Molecular and Cell Biology, Proteos, Singapore.</nlm:affiliation><country xml:lang="fr">Singapour</country><wicri:regionArea>Institute of Molecular and Cell Biology, Proteos</wicri:regionArea><wicri:noRegion>Proteos</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Xiao Bo" sort="Liu, Xiao Bo" uniqKey="Liu X" first="Xiao Bo" last="Liu">Xiao Bo Liu</name></author><author><name sortKey="Fang, Shou Guo" sort="Fang, Shou Guo" uniqKey="Fang S" first="Shou Guo" last="Fang">Shou Guo Fang</name></author><author><name sortKey="Tay, Felicia P L" sort="Tay, Felicia P L" uniqKey="Tay F" first="Felicia P L" last="Tay">Felicia P L. Tay</name></author><author><name sortKey="Liu, Ding Xiang" sort="Liu, Ding Xiang" uniqKey="Liu D" first="Ding Xiang" last="Liu">Ding Xiang Liu</name></author></analytic><series><title level="j">PloS one</title><idno type="eISSN">1932-6203</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Amino Acid Substitution</term><term>Animals</term><term>Blotting, Western</term><term>Cell Fusion</term><term>Cells, Cultured</term><term>Coronavirus (pathogenicity)</term><term>Electroporation</term><term>Flow Cytometry</term><term>Fluorescent Antibody Technique</term><term>Membrane Glycoproteins (chemistry)</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (physiology)</term><term>Molecular Sequence Data</term><term>Mutagenesis, Site-Directed</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Sequence Homology, Amino Acid</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (chemistry)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules cultivées</term><term>Coronavirus (pathogénicité)</term><term>Cytométrie en flux</term><term>Données de séquences moléculaires</term><term>Fusion cellulaire</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires ()</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (physiologie)</term><term>Mutagenèse dirigée</term><term>Protéines de l'enveloppe virale ()</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (physiologie)</term><term>RT-PCR</term><term>Similitude de séquences d'acides aminés</term><term>Substitution d'acide aminé</term><term>Séquence d'acides aminés</term><term>Technique d'immunofluorescence</term><term>Technique de Western</term><term>Électroporation</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Amino Acid Substitution</term><term>Animals</term><term>Blotting, Western</term><term>Cell Fusion</term><term>Cells, Cultured</term><term>Electroporation</term><term>Flow Cytometry</term><term>Fluorescent Antibody Technique</term><term>Molecular Sequence Data</term><term>Mutagenesis, Site-Directed</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Sequence Homology, Amino Acid</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules cultivées</term><term>Cytométrie en flux</term><term>Données de séquences moléculaires</term><term>Fusion cellulaire</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Mutagenèse dirigée</term><term>Protéines de l'enveloppe virale</term><term>RT-PCR</term><term>Similitude de séquences d'acides aminés</term><term>Substitution d'acide aminé</term><term>Séquence d'acides aminés</term><term>Technique d'immunofluorescence</term><term>Technique de Western</term><term>Électroporation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Coronavirus host and cell specificities are determined by specific interactions between the viral spike (S) protein and host cell receptor(s). Avian coronavirus infectious bronchitis (IBV) has been adapted to embryonated chicken eggs, primary chicken kidney (CK) cells, monkey kidney cell line Vero, and other human and animal cells. Here we report that acquisition of the cell-cell fusion activity by amino acid mutations in the S protein determines the infectivity of IBV in cultured cells. Expression of S protein derived from Vero- and CK-adapted strains showed efficient induction of membrane fusion. However, expression of S protein cloned from the third passage of IBV in chicken embryo (EP3) did not show apparent syncytia formation. By construction of chimeric S constructs and site-directed mutagenesis, a point mutation (L857-F) at amino acid position 857 in the heptad repeat 1 region of S protein was shown to be responsible for its acquisition of the cell-cell fusion activity. Furthermore, a G405-D point mutation in the S1 domain, which was acquired during further propagation of Vero-adapted IBV in Vero cells, could enhance the cell-cell fusion activity of the protein. Re-introduction of L857 back to the S gene of Vero-adapted IBV allowed recovery of variants that contain the introduced L857. However, compensatory mutations in S1 and some distant regions of S2 were required for restoration of the cell-cell fusion activity of S protein carrying L857 and for the infectivity of the recovered variants in cultured cells. This study demonstrates that acquisition of the cell-cell fusion activity in S protein determines the selection and/or adaptation of a coronavirus from chicken embryo to cultured cells of human and animal origins.</div></front></TEI><affiliations><list><country><li>Singapour</li></country></list><tree><noCountry><name sortKey="Fang, Shou Guo" sort="Fang, Shou Guo" uniqKey="Fang S" first="Shou Guo" last="Fang">Shou Guo Fang</name><name sortKey="Liu, Ding Xiang" sort="Liu, Ding Xiang" uniqKey="Liu D" first="Ding Xiang" last="Liu">Ding Xiang Liu</name><name sortKey="Liu, Xiao Bo" sort="Liu, Xiao Bo" uniqKey="Liu X" first="Xiao Bo" last="Liu">Xiao Bo Liu</name><name sortKey="Tay, Felicia P L" sort="Tay, Felicia P L" uniqKey="Tay F" first="Felicia P L" last="Tay">Felicia P L. Tay</name></noCountry><country name="Singapour"><noRegion><name sortKey="Yamada, Yoshiyuki" sort="Yamada, Yoshiyuki" uniqKey="Yamada Y" first="Yoshiyuki" last="Yamada">Yoshiyuki Yamada</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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